Decoding optimal ligand design for multicomponent condensates

Sarasi Galagedera.

SASDRM7 – M1-linked tetraubiquitin, M1(1-74)-Ub4

Polyubiquitin-B
MWI(0) 32 kDa
MWexpected 34 kDa
VPorod 43 nm3
log I(s) 2.01×10-2 2.01×10-3 2.01×10-4 2.01×10-5
Polyubiquitin-B small angle scattering data  s, nm-1
ln I(s)
Polyubiquitin-B Guinier plot ln 2.01×10-2 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Polyubiquitin-B Kratky plot 1.104 0 √3 sRg
p(r)
Polyubiquitin-B pair distance distribution function Rg: 3.4 nm 0 Dmax: 12.2 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of M1-linked tetraubiquitin, M1(1-74)-Ub4 in 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH 6.8 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 7.09 mg/ml was measured at 20°C. 13 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Polyubiquitin-B (M1(1-74)-Ub4)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   33.9 kDa
 
UniProt   P0CG47 (1-74)
Sequence   FASTA