Solution structure of poly(UG) RNA

Escobar C, Petersen R, Tonelli M, Fan L, Henzler-Wildman K, Butcher S, Journal of Molecular Biology :168340 (2023) DOI

SASDRT7 – poly(GU) RNA with 12 GU repeats - (GU)12 RNA

polyGU RNA - (GU)12
MWexperimental 7 kDa
MWexpected 8 kDa
VPorod 11 nm3
log I(s) 4.43×10-2 4.43×10-3 4.43×10-4 4.43×10-5
polyGU RNA - (GU)12 small angle scattering data  s, nm-1
ln I(s)
polyGU RNA - (GU)12 Guinier plot ln 4.44×10-2 Rg: 1.4 nm 0 (1.4 nm)-2 s2
(sRg)2I(s)/I(0)
polyGU RNA - (GU)12 Kratky plot 1.104 0 √3 sRg
p(r)
polyGU RNA - (GU)12 pair distance distribution function Rg: 1.4 nm 0 Dmax: 5.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
polyGU RNA - (GU)12 OTHER model
log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of (GU)12 RNA in 20 mM HEPES, 150 mM KCl, pH 7 were collected on the 12-ID-B beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Eiger 2S detector at a sample-detector distance of 1.9 m and at a wavelength of λ = 0.0932 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.3 and 20 mg/ml were measured at 22°C. 60 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

polyGU RNA - (GU)12 ((GU)12)
Mol. type   RNA
Organism   synthetic RNA
Olig. state   Monomer
Mon. MW   7.8 kDa
Sequence   FASTA