| MWexperimental | 16 | kDa |
| MWexpected | 17 | kDa |
| VPorod | 28 | nm3 |
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log I(s)
2.67×103
2.67×102
2.67×101
2.67×100
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s, nm-1
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Structure of the N-terminal didomain d1_d2 of the Thrombospondin type-1 domain-containing 7A
Bochel A, Mortensen S, Seifert L, Hengel F, Jeffries C, Chojnowski G, Kretz O, Huber T, Tomas N, Wilmanns M, (2023) DOISASDRZ6 – D1-D2 N-terminal domains of thrombospondin type-1 domain-containing protein 7A
Thrombospondin type-1 domain-containing protein 7A|
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Fits and models
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Synchrotron SAXS data from solutions of the D1-D2 N-terminal domains from thrombospondin type-1 domain-containing protein 7A in 25 mM Tris, 150 mM NaCl, 5 mM NaNO3, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 37.00 μl sample at 4.7 mg/ml was injected at a 0.35 ml/min flow rate onto a GE Superdex 75 Increase 5/150 column at 20°C. 48 successive 0.250 second frames were collected through the SEC-elution peak and processed using CHROMIXS. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Additional SEC-SAXS data (unsubtracted data frames), CHROMIXS Rg correlations, and models are made available in the full entry zip archive |
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