Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA

Stephanie Hicks.

SASDS23 – single stranded 9-mer RNA: tristetraprolin TZF domain optimal target sequence, pH 8 at 14 mg/mL

single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence
MWexperimental 3 kDa
MWexpected 3 kDa
VPorod 5 nm3
log I(s) 3.42×100 3.42×10-1 3.42×10-2 3.42×10-3
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence small angle scattering data  s, nm-1
ln I(s)
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Guinier plot ln 3.42×100 Rg: 1.0 nm 0 (1.0 nm)-2 s2
(sRg)2I(s)/I(0)
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Kratky plot 1.104 0 √3 sRg
p(r)
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence pair distance distribution function Rg: 1.0 nm 0 Dmax: 3.2 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of single stranded 9-mer RNA in 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH 8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 14.00 mg/ml was measured at 10°C. 32 successive 0.300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Data are severely truncated at higher angle.

single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence (9-mer RNA)
Mol. type   RNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   2.8 kDa
Sequence   FASTA