| MWexperimental | 63 | kDa |
| MWexpected | 61 | kDa |
| VPorod | 92 | nm3 |
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log I(s)
1.19×10-2
1.19×10-3
1.19×10-4
1.19×10-5
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s, nm-1
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Unravelling structural dynamics of Dengue virus 3' UTR and its impact on viral life cycle
Trushar Patel.SASDS59 – G4 dumbbell RNA mutant - 3' UTR of Dengue virus
G4 dumbbell Mut - 3' untranslated region (3' UTR) Dengue virus|
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Fits and models
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Synchrotron SAXS data from solutions of mutant G4 dumbbell RNA - 3' UTR of Dengue virus - in 20 mM HEPES, 100 mM KCl, 0.01 mM EDTA, pH 7 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.094 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 40.00 μl sample at 1.5 mg/ml was injected at a 0.16 ml/min flow rate onto a Shodex KW403-4F column at 15°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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