Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase.

Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M, J Struct Biol 215(4):108028 (2023) Europe PMC

SASDSB2 – Sulfite reductase flavoprotein crosslinked reduced with 10 molar equivalents sodium dithionite

Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C)
MWexperimental 585 kDa
MWexpected 566 kDa
VPorod 827 nm3
log I(s) 4.94×100 4.94×10-1 4.94×10-2 4.94×10-3
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) small angle scattering data  s, nm-1
ln I(s)
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) Guinier plot ln 4.95×100 Rg: 8.0 nm 0 (8.0 nm)-2 s2
(sRg)2I(s)/I(0)
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) Kratky plot 1.104 0 √3 sRg
p(r)
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) pair distance distribution function Rg: 8.1 nm 0 Dmax: 25.3 nm

Data validation

There are no models related to this curve.

SANS data from solutions of sulfite reductase crosslinked octamer reduced with 10 molar equivalents sodium dithionite in 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH 7.8 were collected using the EQ-SANS (BL-6) spallation neutron source (Oak Ridge, Tennessee, USA). This sample is an octamer of His-tagged sulfite reductase flavoprotein subunits. Two instrument configurations were used: 4 m sample-to-detector distance with 1.0 nm wavelength band, 2.5 m sample-to-detector distance with 0.25 nm wavelength band. Sample concentration: 3 mg/mL. Neutron exposure time: 1 hour / instrument configuration. Sample temperature: 8°C.

#

Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Octamer
Mon. MW   70.7 kDa
 
UniProt   P38038 (1-599)
Sequence   FASTA