Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase.

Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M, J Struct Biol 215(4):108028 (2023) Europe PMC

SASDSC2 – Sulfite reductase truncated linker (ΔAAPSQS) flavoprotein reduced with 10 molar equivalents sodium dithionite

Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217)
MWexperimental 646 kDa
MWexpected 526 kDa
VPorod 952 nm3
log I(s) 2.57×100 2.57×10-1 2.57×10-2 2.57×10-3
Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) small angle scattering data  s, nm-1
ln I(s)
Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) Guinier plot ln 2.57×100 Rg: 7.6 nm 0 (7.6 nm)-2 s2
(sRg)2I(s)/I(0)
Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) Kratky plot 1.104 0 √3 sRg
p(r)
Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) pair distance distribution function Rg: 7.9 nm 0 Dmax: 25 nm

Data validation

There are no models related to this curve.

SANS data from solutions of sulfite reductase truncated linker (ΔAAPSQS) flavoprotein octamer in 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH 7.8 were collected using the EQ-SANS (BL-6) spallation neutron source (Oak Ridge, Tennessee, USA). This sample is an octamer of His-tagged sulfite reductase flavoprotein with 6 amino acid deletion in linker (ΔAAPSQS). Two instrument configurations were used: 4 m sample-to-detector distance with 1.0 nm wavelength band, 2.5 m sample-to-detector distance with 0.25 nm wavelength band. Sample concentration: 3 mg/mL. Neutron exposure time: 1 hour / instrument configuration. Sample temperature: 8°C.

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Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Octamer
Mon. MW   65.7 kDa
 
UniProt   P38038 (1-599)
Sequence   FASTA