Synchrotron SAXS
data from solutions of
Human Ubiquilin-2, ∆STI1-2 domain deletion (UBQLN2∆STI1-2)
in
20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH 6.8
were collected
on the
BioCAT 18ID beam line
at the Advanced Photon Source (APS), Argonne National Laboratory storage ring
(Lemont, IL, USA)
using a Pilatus3 X 1M detector
at a sample-detector distance of 3.7 m and
at a wavelength of λ = 0.1033 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample
at 1.9 mg/ml was injected at a 0.60 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 20°C.
26 successive
0.500 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Here the STI1-2 domain refers to the second STI1 module of the protein. This module is annotated in UniProt Q9UHD9 as encompassing STI1 3 (amino acids 379-426) and STI1 4 (amino acids 430-462).
s, nm-1
