The dimerized pentraxin-like domain of the adhesion G protein-coupled receptor 112 (ADGRG4) suggests function in sensing mechanical forces.

Kieslich B, Weiße RH, Brendler J, Ricken A, Schöneberg T, Sträter N, J Biol Chem :105356 (2023) Europe PMC

SASDSG8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 1.07 mg/mL

Adhesion G-protein coupled receptor G4
MWexperimental 37 kDa
MWexpected 55 kDa
VPorod 70 nm3
log I(s) 2.88×10-2 2.88×10-3 2.88×10-4 2.88×10-5
Adhesion G-protein coupled receptor G4 small angle scattering data  s, nm-1
ln I(s)
Adhesion G-protein coupled receptor G4 Guinier plot ln 2.89×10-2 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Adhesion G-protein coupled receptor G4 Kratky plot 1.104 0 √3 sRg
p(r)
Adhesion G-protein coupled receptor G4 pair distance distribution function Rg: 2.9 nm 0 Dmax: 9.0 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
log I(s)
 s, nm-1
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
log I(s)
 s, nm-1
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Adhesion G-protein coupled receptor G4 EOM/RANCH model
Synchrotron SAXS data from solutions of Adhesion G protein-coupled receptor G4 PTX-like domain in 20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123987 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.07 mg/ml was measured at 35.2°C. 23 successive 0.195 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Dmax appears under-estimated.

Adhesion G-protein coupled receptor G4 (G4-PTX)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   27.4 kDa
 
UniProt   Q8IZF6 (26-240)
Sequence   FASTA