Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation.

Sarasi Galagedera.

SASDSH6 – Human Ubiquilin-2, PXX domain deletion (UBQLN2∆PXX)

Ubiquilin-2 (∆487-538)
MWI(0) 116 kDa
MWexpected 122 kDa
VPorod 288 nm3
log I(s) 1.90×100 1.90×10-1 1.90×10-2 1.90×10-3
Ubiquilin-2 (∆487-538) small angle scattering data  s, nm-1
ln I(s)
Ubiquilin-2 (∆487-538) Guinier plot ln 1.91×100 Rg: 4.8 nm 0 (4.8 nm)-2 s2
(sRg)2I(s)/I(0)
Ubiquilin-2 (∆487-538) Kratky plot 1.104 0 √3 sRg
p(r)
Ubiquilin-2 (∆487-538) pair distance distribution function Rg: 5.0 nm 0 Dmax: 19.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Human Ubiquilin-2, PXX domain deletion (UBQLN2∆PXX) in 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH 6.8 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample at 1.9 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 24 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Ubiquilin-2 (∆487-538) (UBQLN2∆PXX)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   61.1 kDa
 
UniProt   Q9UHD9 (1-624)
Sequence   FASTA