| MWI(0) | 10 | kDa |
| MWexpected | 8 | kDa |
| VPorod | 27 | nm3 |
|
log I(s)
9.97×100
9.97×10-1
9.97×10-2
9.97×10-3
|
s, nm-1
|
Conformational Disorder Analysis of the Conditionally Disordered Protein CP12 from Arabidopsis thaliana in Its Different Redox States
Del Giudice A Gurrieri L, Galantini L, Fanti S, Trost P, Sparla F, Fermani S, International Journal of Molecular Sciences 24(11):9308 (2023) DOISASDSN2 – AtCP12-2 in a reduced form
CP12-2 (D77G)|
|
|
Fits and models
|
Rg, nm
|
|
Synchrotron SAXS data from solutions of AtCP12-2 in a reduced form in 25 mM potassium phosphate, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.3 and 5.6 mg/ml were measured at 4°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Tags:
idp
|
|
|||||||||||||||||||||||||||