| MWexperimental | 113 | kDa |
| MWexpected | 126 | kDa |
| VPorod | 186 | nm3 |
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log I(s)
1.32×102
1.32×101
1.32×100
1.32×10-1
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s, nm-1
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Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy.SASDST7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the apo form in the presence of 4 molar excess of L-Tyrosine and ATP analog (AMPPCP)
Gramicidin S synthase 1 (W239S)|
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Fits and models
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Synchrotron SAXS data from solutions of Gramicidin Synthetase 1 (GrsA) W239S mutant in the apo form in the presence of L-Tyrosine and AMPPCP in 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 7.9°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The apo form of GrsA-W239S exists in an ensemble of 2 states as determined by bilboMD even in presence of L-Tyrosine and non-hydrolyzable ATP analog (AMPPCP; https://pubchem.ncbi.nlm.nih.gov/compound/AMP-PCP-_disodium). Individually, the compact form is present as 74% of the representative species with Dmax of 14.4 nm and Rg of 4.10 nm, while the elongated form was present as 24% of the ensemble with Dmax of 15.9 nm and Rg of 4.76 nm. The atomic models displayed in this entry represent the compact form (top) and elongated form (bottom). |
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