Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA

Stephanie Hicks.

SASDSW2 – spikemoss tandem zinc finger (TZF) domain, pH 8 at 4.1 mg/mL

C3H1-type domain-containing protein
MWexperimental 8 kDa
MWexpected 8 kDa
VPorod 14 nm3
log I(s) 4.95×100 4.95×10-1 4.95×10-2 4.95×10-3
C3H1-type domain-containing protein small angle scattering data  s, nm-1
ln I(s)
C3H1-type domain-containing protein Guinier plot ln 4.96×100 Rg: 1.7 nm 0 (1.7 nm)-2 s2
(sRg)2I(s)/I(0)
C3H1-type domain-containing protein Kratky plot 1.104 0 √3 sRg
p(r)
C3H1-type domain-containing protein pair distance distribution function Rg: 1.9 nm 0 Dmax: 7.1 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of spikemoss tandem zinc finger (TZF) domain in 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH 8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS, Berkeley, CA, USA) using a MAR 165 CCD detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.10 mg/ml was measured at 10°C. 32 successive 0.300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Data are severely truncated at higher angle.

C3H1-type domain-containing protein (smTZF domain)
Mol. type   Protein
Organism   Selaginella moellendorffii
Olig. state   Monomer
Mon. MW   8.1 kDa
 
UniProt   D8SB57 (10-76)
Sequence   FASTA