BCL7 proteins, novel subunits of the mammalian SWI/SNF complex, bind the nucleosome core particle

Asgar Abbas kazrani.

SASDSW5 – B cell lymphoma 7 protein family member A (BCL7A)

Isoform 2 of B-cell CLL/lymphoma 7 protein family member A
MWI(0) 38 kDa
MWexpected 25 kDa
log I(s) 1.05×10-2 1.05×10-3 1.05×10-4 1.05×10-5
Isoform 2 of B-cell CLL/lymphoma 7 protein family member A small angle scattering data  s, nm-1
ln I(s)
Isoform 2 of B-cell CLL/lymphoma 7 protein family member A Guinier plot ln 1.06×10-2 Rg: 4.8 nm 0 (4.8 nm)-2 s2
(sRg)2I(s)/I(0)
Isoform 2 of B-cell CLL/lymphoma 7 protein family member A Kratky plot 1.104 0 3 sRg
Dmax: 28.8 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of B cell lymphoma 7 protein family member A (BCL7A) in 20 mM Tris-HCl , 50 mM KCl, 1 mM DTT, pH 8 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 0.5 mg/ml was injected at a 0.30 ml/min flow rate onto a Cytiva Superose 6 Increase 3.2/300 column at 15°C. 780 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Isoform 2 of B-cell CLL/lymphoma 7 protein family member A (BCL7A)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   25.0 kDa
 
UniProt   Q4VC05-2 (1-231)
Sequence   FASTA