| MWI(0) | 288 | kDa |
| MWexpected | 224 | kDa |
| VPorod | 400 | nm3 |
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log I(s)
2.83×10-1
2.83×10-2
2.83×10-3
2.83×10-4
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s, nm-1
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BCL7 proteins, novel subunits of the mammalian SWI/SNF complex, bind the nucleosome core particle
Asgar Abbas kazrani.SASDSY5 – Nucleosome (NCP) in complex with B cell lymphoma 7 protein family member A (BCL7A)
Isoform 2 of B-cell CLL/lymphoma 7 protein family member AHistone H3.2
Histone H4
Histone H2A
Histone H2B 1.1
Widom 601 DNA sequence
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Fits and models
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Synchrotron SAXS data from solutions of a nucleosome core particle (histones plus Widom 601 DNA) in complex with B cell lymphoma 7 protein family member A in 20 mM Tris-HCl , 50 mM KCl, 1 mM DTT, pH 8 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 0.5 mg/ml was injected at a 0.30 ml/min flow rate onto a Agilent Bio SEC-5, 500Å column at 15°C. 780 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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