Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes.

S├╝lzen H, Volkov AN, Geens R, Zahedifard F, Stijlemans B, Zoltner M, Magez S, Sterckx YG, Zoll S, PLoS Pathog 20(4):e1012186 (2024) Europe PMC

SASDTA5 – Trypanosoma brucei gambiense variable surface glycoprotein LiTat 1.5

Variable surface glycoprotein LiTat 1.5
MWexperimental 101 kDa
MWexpected 98 kDa
VPorod 170 nm3
log I(s) 1.79×10-1 1.79×10-2 1.79×10-3 1.79×10-4
Variable surface glycoprotein LiTat 1.5 small angle scattering data  s, nm-1
ln I(s)
Variable surface glycoprotein LiTat 1.5 Guinier plot ln 1.79×10-1 Rg: 4.4 nm 0 (4.4 nm)-2 s2
(sRg)2I(s)/I(0)
Variable surface glycoprotein LiTat 1.5 Kratky plot 1.104 0 3 sRg
p(r)
Variable surface glycoprotein LiTat 1.5 pair distance distribution function Rg: 4.7 nm 0 Dmax: 17.2 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Variable surface glycoprotein LiTat 1.5 BILBOMD model
Variable surface glycoprotein LiTat 1.5 BILBOMD model
Variable surface glycoprotein LiTat 1.5 BILBOMD model
Variable surface glycoprotein LiTat 1.5 BILBOMD model
Variable surface glycoprotein LiTat 1.5 BILBOMD model

Synchrotron SAXS data from solutions of Trypanosoma brucei gambiense variable surface glycoprotein LiTat 1.5 in 50 mM Tris-HCl, 500 mM NaCl, pH 7.5 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 10 mg/ml was injected at a 0.20 ml/min flow rate onto a Shodex KW404-4F column at 20°C. 1000 successive 0.750 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Variable surface glycoprotein LiTat 1.5 (TbbVSGLiTat1.5 VSG)
Mol. type   Protein
Organism   Trypanosoma brucei gambiense
Olig. state   Dimer
Mon. MW   49.1 kDa
 
UniProt   E7EDN2 (27-483)
Sequence   FASTA