SAXS/WAXS data of conformationally flexible ribose binding protein

Choudhury J, Yonezawa K, Anu A, Shimizu N, Chaudhuri B, Data in Brief :109932 (2023) DOI

SASDTD2 – WAXS data of poly-histidine tagged Ribose binding protein (17 mg/mL) from Escherichia coli in the presence of 5 mM ribose

Ribose import binding protein RbsB
Ribose
MWexperimental 21 kDa
MWexpected 30 kDa
VPorod 25 nm3
log I(s) 1.06×100 1.06×10-1 1.06×10-2 1.06×10-3
Ribose import binding protein RbsB Ribose small angle scattering data  s, nm-1
ln I(s)
Ribose import binding protein RbsB Ribose Guinier plot ln 1.06×100 Rg: 1.9 nm 0 (1.9 nm)-2 s2
(sRg)2I(s)/I(0)
Ribose import binding protein RbsB Ribose Kratky plot 1.104 0 3 sRg
Dmax: 10.7 nm

Data validation


There are no models related to this curve.

Synchrotron WAXS data of poly-histidine tagged Ribose binding protein from Escherichia coli in 50 mM Tris, 50 mM NaCl, 10% glycerol, 5 mM ribose, pH 7 were collected on the BL-15A2 beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK) storage ring (Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.121297 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 17.00 mg/ml was measured at 22°C. 60 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Note: The experimental molecular weight, Rg, Dmax and Porod volume are not valid parameters in this case, and are provided only as approximate estimates.

Ribose import binding protein RbsB
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Monomer
Mon. MW   29.7 kDa
 
UniProt   P02925 (25-296)
Sequence   FASTA
 
Ribose
Mol. type   Other
Olig. state   Monomer
Mon. MW   0.2 kDa
Chemical formula