Solution structure and functional characterization of an avian picornavirus 2AH/NC protein

Anna Wehlin.

SASDTT2 – Turkey Avisivirus 2A3 (TuAsV 2A3) polyprotein fragment at pH 7.4

Genome polyprotein
MWexperimental 13 kDa
MWexpected 13 kDa
VPorod 24 nm3
log I(s) 1.37×10-2 1.37×10-3 1.37×10-4 1.37×10-5
Genome polyprotein small angle scattering data  s, nm-1
ln I(s)
Genome polyprotein Guinier plot ln 1.37×10-2 Rg: 1.6 nm 0 (1.6 nm)-2 s2
(sRg)2I(s)/I(0)
Genome polyprotein Kratky plot 1.104 0 √3 sRg
p(r)
Genome polyprotein pair distance distribution function Rg: 1.5 nm 0 Dmax: 4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Genome polyprotein ALPHAFOLD model
Synchrotron SAXS data from solutions of TuAsV 2A3 in 20 mM HEPES, 150 mM NaCl, 2 mM TECP, 1% glycerol, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 16.00 μl sample at 9.6 mg/ml was injected at a 0.30 ml/min flow rate onto a GE Superdex 75 Increase 5/150 column at 25°C. 40 successive 1 second frames were collected through the SEC elution peak of the sample. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Genome polyprotein (TuAsV 2A3)
Mol. type   Protein
Organism   Turkey avisivirus
Olig. state   Monomer
Mon. MW   13.3 kDa
 
UniProt   M1L6N7 (944-1066)
Sequence   FASTA