B56δ long-disordered arms form a dynamic PP2A regulation interface coupled with global allostery and Jordan's syndrome mutations.

Wu CG, Balakrishnan VK, Merrill RA, Parihar PS, Konovolov K, Chen YC, Xu Z, Wei H, Sundaresan R, Cui Q, Wadzinski BE, Swingle MR, Musiyenko A, Chung WK, Honkanen RE, Suzuki A, Huang X, Strack S, Xing Y, Proc Natl Acad Sci U S A 121(1):e2310727120 (2024) Europe PMC

SASDTW2 – Protein phosphatase 2A (PP2A)-B56δ holoenzyme wildtype

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H)
Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
MWexperimental 169 kDa
MWexpected 171 kDa
VPorod 266 nm3
log I(s) 3.32×100 3.32×10-1 3.32×10-2 3.32×10-3
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H) Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform small angle scattering data  s, nm-1
ln I(s)
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H) Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Guinier plot ln 3.32×100 Rg: 4.0 nm 0 (4.0 nm)-2 s2
(sRg)2I(s)/I(0)
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H) Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Kratky plot 1.104 0 3 sRg
p(r)
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H) Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform pair distance distribution function Rg: 3.9 nm 0 Dmax: 12 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of PP2A-B56δ holoenzyme wildtype in a buffer (25 mM Tris-HCl pH 8.0, 150 mM NaCl, 50 µM MnCl2) were collected on the BioCAT 18-ID-D beamline at the Advanced Photon Source (APS) (Chicago, IL, USA) using a Pilatus3 X 1M detector (Dectris) at a sample-detector distance of ~3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC)-SAXS was employed. The SEC parameters were as follows: A 550 μl sample at 1.5 mg/ml was injected at a 0.70 ml/min flow rate onto a Superdex 200 10/300 column (GE healthcare) at 22°C. 2000 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (Scaffold A subunit)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   65.5 kDa
 
UniProt   P30153 (1-589)
Sequence   FASTA
 
Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H) (regulatory B subunit)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   70.1 kDa
 
UniProt   Q14738-1 (1-602)
Sequence   FASTA
 
Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (Catalytic C subunit)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   35.9 kDa
 
UniProt   P67775-1 (1-309)
Sequence   FASTA