Wu CG,
Balakrishnan VK,
Merrill RA,
Parihar PS,
Konovolov K,
Chen YC,
Xu Z
Wei H,
Sundaresan R,
Cui Q,
Wadzinski BE,
Swingle MR,
Musiyenko A,
Chung WK,
Honkanen RE,
Suzuki A,
Huang X,
Strack S,
Xing Y,
Proc Natl Acad Sci U S A
121(1):e2310727120
(2024)
Europe PMC
SASDTW2 – Protein phosphatase 2A (PP2A)-B56δ holoenzyme wildtype
Synchrotron SAXS data from solutions of PP2A-B56δ holoenzyme wildtype in a buffer (25 mM Tris-HCl pH 8.0, 150 mM NaCl, 50 µM MnCl2) were collected on the BioCAT 18-ID-D beamline at the Advanced Photon Source (APS) (Chicago, IL, USA) using a Pilatus3 X 1M detector (Dectris) at a sample-detector distance of ~3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC)-SAXS was employed. The SEC parameters were as follows: A 550 μl sample at 1.5 mg/ml was injected at a 0.70 ml/min flow rate onto a Superdex 200 10/300 column (GE healthcare) at 22°C. 2000 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
