Allovalent scavenging of activation domains in the transcription factor ANAC013 gears transcriptional regulation.

Delaforge E Due AD, Theisen FF, Morffy N, O'Shea C, Blackledge M, Strader LC, Skriver K, Kragelund BB, Nucleic Acids Res 53(4) (2025) Europe PMC

SASDU27 – The DNA binding domain and intrinsically disordered region of NAC domain-containing protein 13 (ANAC013; amino acids 1-274)

NAC domain-containing protein 13
MWexperimental 72 kDa
MWexpected 63 kDa
VPorod 118 nm3
log I(s) 4.31×10-2 4.31×10-3 4.31×10-4 4.31×10-5
NAC domain-containing protein 13 small angle scattering data  s, nm-1
ln I(s)
NAC domain-containing protein 13 Guinier plot ln 4.31×10-2 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
NAC domain-containing protein 13 Kratky plot 1.104 0 √3 sRg
p(r)
NAC domain-containing protein 13 pair distance distribution function Rg: 4.0 nm 0 Dmax: 17 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of ANAC013 (amino acids 1-274) in 20 mM HEPES, 150 mM NaCl, 5 mM NaNO3, 5 mM TCEP, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123981 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 70.00 μl sample at 8.6 mg/ml was injected at a 0.65 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 2220 successive 1 second frames were collected through the entire SEC elution; 18 sample frames were used for averaging. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

NAC domain-containing protein 13 (ANAC013 (DBD+IDR))
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Dimer
Mon. MW   31.6 kDa
 
UniProt   F4IED2 (1-274)
Sequence   FASTA