Synchrotron SAXS data from solutions of ANAC013 (amino acids 161-274) in 20 mM HEPES, 150 mM NaCl, 5 mM NaNO3, 5 mM TCEP, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123981 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 70.00 μl sample at 9.1 mg/ml was injected at a 0.65 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 2220 successive 1 second frames were collected through the entire SEC elution; 36 sample frames were used for averaging. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
