Chromochloris zofingiensis Hexokinase-1 (HXK-1)

Estella Yee.

SASDU38 – Chromochloris zofingiensis Hexokinase-1 (37-499) wild-type

Chromochloris zofingiensis Hexokinase-1
MWexperimental 44 kDa
MWexpected 55 kDa
VPorod 72 nm3
log I(s) 3.53×101 3.53×100 3.53×10-1 3.53×10-2
Chromochloris zofingiensis Hexokinase-1 small angle scattering data  s, nm-1
ln I(s)
Chromochloris zofingiensis Hexokinase-1 Guinier plot ln 3.53×101 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
Chromochloris zofingiensis Hexokinase-1 Kratky plot 1.104 0 √3 sRg
p(r)
Chromochloris zofingiensis Hexokinase-1 pair distance distribution function Rg: 2.5 nm 0 Dmax: 7.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of Chromochloris zofingiensis Hexokinase-1 (37-499) wild-type in 50 mM HEPES, 150 mM NaCl, 10 mM MgCl2, 1 mM TCEP, pH 8 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) using a Pilatus3 X 1M , Pilatus3 X 900K dual detectors at a sample-detector distance of 3.7 m (SAXS) and 0.345 m (WAXS) at a wavelength of λ = 0.08203 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 95.00 μl sample was injected at a 0.35 ml/min flow rate onto a Biozen 3 µm dSEC-2, 200 Å column . Eight successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. Cell temperature = UNKNOWN

Cell temperature = UNKNOWN

Chromochloris zofingiensis Hexokinase-1 (CzHXK1)
Mol. type   Protein
Organism   Chromochloris zofingiensis
Olig. state   Monomer
Mon. MW   55.2 kDa
Sequence   FASTA