SASDU58 – Chromochloris zofingiensis Hexokinase-1 (37-499) E291W variant with 200 mM glucose

Chromochloris zofingiensis Hexokinase-1 (E291W)

MW^experimental	49	kDa
MW^expected	55	kDa
V^Porod	87	nm³

log I(s) 2.42×10¹ 2.42×10⁰ 2.42×10^-1 2.42×10^-2

Chromochloris zofingiensis Hexokinase-1 (E291W) small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Chromochloris zofingiensis Hexokinase-1 (E291W) Guinier plot

ln 2.42×10¹ R_g: 2.7 nm 0 (2.7 nm)^-2 s²

(sR_g)²I(s)/I(0)

Chromochloris zofingiensis Hexokinase-1 (E291W) Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 2.7 nm 0 D_max: 8.6 nm

Data validation

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Synchrotron SAXS data from solutions of Chromochloris zofingiensis Hexokinase-1 (37-499) wild-type in 50 mM HEPES, 150 mM NaCl, 10 mM MgCl2, 1 mM TCEP, pH 8 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) using a Pilatus3 X 1M , Pilatus3 X 900K dual detectors at a sample-detector distance of 3.737 m (SAXS) and 0.345 m (WAXS) at a wavelength of λ = 0.08203 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 95.00 μl sample was injected at a 0.35 ml/min flow rate onto a Biozen 3 µm dSEC-2, 200 Å column . Eight successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. Cell temperature =UNKNOWN,

Cell temperature = UNKNOWN

Chromochloris zofingiensis Hexokinase-1 (E291W) (CzHXK1 E291W)
Mol. type		Protein
Organism		Chromochloris zofingiensis
Olig. state		Monomer
Mon. MW		55.3 kDa
Sequence		FASTA