Chromochloris zofingiensis Hexokinase-1 (HXK-1)

Estella Yee.

SASDU68 – Chromochloris zofingiensis Hexokinase-1 (37-499) E291W variant

Chromochloris zofingiensis Hexokinase-1 (E291W)
MWexperimental 45 kDa
MWexpected 55 kDa
VPorod 75 nm3
log I(s) 1.28×101 1.28×100 1.28×10-1 1.28×10-2
Chromochloris zofingiensis Hexokinase-1 (E291W) small angle scattering data  s, nm-1
ln I(s)
Chromochloris zofingiensis Hexokinase-1 (E291W) Guinier plot ln 1.29×101 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
Chromochloris zofingiensis Hexokinase-1 (E291W) Kratky plot 1.104 0 √3 sRg
p(r)
Chromochloris zofingiensis Hexokinase-1 (E291W) pair distance distribution function Rg: 2.6 nm 0 Dmax: 8.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of Chromochloris zofingiensis Hexokinase-1 (37-499) wild-type in 50 mM HEPES, 150 mM NaCl, 10 mM MgCl2, 1 mM TCEP, pH 8 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) using a Pilatus3 X 1M , Pilatus3 X 900K dual detectors at a sample-detector distance of 3.685 m (SAXS) and 0.345 m (WAXS) at a wavelength of λ = 0.08203 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 95.00 μl sample was injected at a 0.35 ml/min flow rate onto a Biozen 3 µm dSEC-2, 200 Å column . Eight successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. Cell temperature = UNKNOWN Cell temperature = UNKNOWN

Cell temperature = UNKNOWN

Chromochloris zofingiensis Hexokinase-1 (E291W) (CzHXK1 E291W)
Mol. type   Protein
Organism   Chromochloris zofingiensis
Olig. state   Monomer
Mon. MW   55.3 kDa
Sequence   FASTA