MWexperimental | 43 | kDa |
MWexpected | 43 | kDa |
VPorod | 76 | nm3 |
log I(s)
6.82×101
6.82×100
6.82×10-1
6.82×10-2
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Synchrotron SAXS data from solutions of ESAG4 venus fly trap domain 2 in 50 mM Tris-HCl, 500 mM NaCl, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 9.9 mg/ml was injected at a 0.30 ml/min flow rate onto a column at 20°C. 500 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
SEC column: UNKNOWN. |
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