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Synchrotron SAXS data from solutions of insulin-like growth factor 2 mRNA-binding protein 2 (IMP2) in 20 mM HEPES, 2 mM MgCl2, 150 mM NaCl, 10% glycerol v/v, 2 mM β-mercaptoethanol, pH 7.4 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II; Brookhaven National Laboratory, Upton, NY, USA) using dual Pilatus3 S 1M/Pilatus3 900 K detectors at a wavelength of λ = 0.11 nm and 1.5 mm path-length (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed and used to separate proteinaceous species. Samples were loaded into a 96-well auto-sampler plate prior to injection into a pre-equilibrated size exclusion-coupled small-angle X-ray scattering (SEC-SAXS) system. An Agilent 1260 Infinity Bio-Inert high-performance liquid chromatography (HPLC) with an auto-sampler was used for sample injection and SEC. The SEC parameters were as follows: A 100.00 μl sample at 5 mg/ml was injected at a 0.35 ml/min flow rate onto a Cytiva Superdex 200 5/150 column at 4°C. Successive 2 second data frames were collected during the course of 15-minute SEC-elution (three frames were selected across the sample elution peak). SAXS/WAXS images were radially integrated before being background subtracted using LIX specific python packages py4xs and lixtools. Subtracted profiles were exported and imported for analysis in BioXTAS RAW (RAW).
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s, nm-1
