Structural basis of the monkeypox virus mRNA cap N7 methyltransferase complex.

Chen A Fang N, Zhang Z, Wen Y, Shen Y, Zhang Y, Zhang L, Zhao G, Ding J, Li J, Emerg Microbes Infect 13(1):2369193 (2024) Europe PMC

SASDUM2 – mRNA capping enzyme MTase

Virus termination factor small subunit
mRNA-capping enzyme catalytic subunit
MWexperimental 67 kDa
MWexpected 68 kDa
VPorod 106 nm3
log I(s) 5.31×101 5.31×100 5.31×10-1 5.31×10-2
Virus termination factor small subunit mRNA-capping enzyme catalytic subunit small angle scattering data  s, nm-1
ln I(s)
Virus termination factor small subunit mRNA-capping enzyme catalytic subunit Guinier plot ln 5.31×101 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Virus termination factor small subunit mRNA-capping enzyme catalytic subunit Kratky plot 1.104 0 √3 sRg
p(r)
Virus termination factor small subunit mRNA-capping enzyme catalytic subunit pair distance distribution function Rg: 2.9 nm 0 Dmax: 10.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Virus termination factor small subunit mRNA-capping enzyme catalytic subunit DAMFILT model
Virus termination factor small subunit mRNA-capping enzyme catalytic subunit DAMMIF model
Synchrotron SAXS data from solutions of mRNA capping enzyme MTase in 20 mM Tris, 150 mM NaCl, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 25°C. 10 successive 6 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The dummy atom models displayed in this entry show: Top - the minimised volume and bead-occupancy 'averaged' shape obtained from the spatial alignment of a ten-member individual model cohort (DAMFILT) and; Bottom - an example of an individual model, with the corresponding individual-model fit to the data (displayed to the left). The ten-member model cohort is available for download in the full entry zip archive.

Virus termination factor small subunit
Mol. type   Protein
Organism   Monkeypox virus (strain Zaire-96-I-16)
Olig. state   Monomer
Mon. MW   33 kDa
 
UniProt   Q8V4X6 (1-287)
Sequence   FASTA
 
mRNA-capping enzyme catalytic subunit
Mol. type   Protein
Organism   Monkeypox virus (strain Zaire-96-I-16)
Olig. state   Monomer
Mon. MW   34.6 kDa
 
UniProt   Q8V4Y7 (546-845)
Sequence   FASTA