| MWI(0) | 10 | kDa |
| MWexpected | 114 | kDa |
| VPorod | 181 | nm3 |
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log I(s)
1.07×101
1.07×100
1.07×10-1
1.07×10-2
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s, nm-1
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Collagenase G
Cody Brazel.SASDUM8 – Collagenase G at pCa 3
Collagenase ColG (E656D, N659T, G836V, D837Y)|
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Fits and models
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Synchrotron SAXS data from solutions of Collagenase G of Hathewaya histolytica at pCa value of 3. pCa = -Log[Ca] in moles/liter. Suspended in 50 mM Tris, 1 mM CaCl2, 100mM NaCl, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Protein concentrations ranging between 1 and 5 mg/ml were measured at 10 °C. 50 successive frames were collected in 10 seconds per well. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
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