|
Synchrotron SAXS data from solutions of Collagenase G of Hathewaya histolytica at pCa value of 7. pCa = -Log[Ca] in moles/liter. Suspended in 50 mM Tris, 1 mM CaCl2, 100mM NaCl, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). EDTA was added to modulate available calcium concentration in solution. Protein concentrations ranging between 1 and 5 mg/ml were measured at 10°C. 50 successive frames were collected in 10 seconds per well. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
EDTA was added for molar calcium concentration of -Log[7].
|
|
s, nm-1
