Sequential conformational transition of ArnB, an archaeal ortholog with Sec23/Sec24 core motif

Essen L, Korf L, Steinchen W, Watad M, Bezold F, Vogt M, Selbach L, Penner A, Tourte M, Hepp S, Albers S, (2024) DOI

SASDUW2 – ArnA-ArnB complex, unphosphorylated

Conserved protein
Conserved protein

MW^experimental	64	kDa
MW^expected	68	kDa
V^Porod	81	nm³

log I(s) 5.51×10¹ 5.51×10⁰ 5.51×10^-1 5.51×10^-2

Conserved protein Conserved protein small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Conserved protein Conserved protein Guinier plot

ln 5.52×10¹ R_g: 4.2 nm 0 (4.2 nm)^-2 s²

(sR_g)²I(s)/I(0)

Conserved protein Conserved protein Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 4.2 nm 0 D_max: 19.2 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.6

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.598
1.004

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Conserved protein Conserved protein DAMMIN model

Synchrotron SAXS data from solutions of the ArnA-ArnB complex in 50 mM Tris, 150 mM NaCl, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) at 20°C using a Pilatus 1M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN. Concentration min = UNKNOWN. Concentration max = UNKNOWN

Conserved protein (ArnA)
Mol. type		Protein
Organism		Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Olig. state		Monomer
Mon. MW		24.0 kDa

UniProt		Q4J9H4 (1-206)
Sequence		FASTA

Conserved protein (ArnB)
Mol. type		Protein
Organism		Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Olig. state		Monomer
Mon. MW		43.7 kDa

UniProt		Q4J9H3 (2-380)
Sequence		FASTA