SAXS data of Mycobacterium tuberculosis SepF-C-terminal domain

Jagrity Choudhury.

SASDUY2 – Polyhistidine tagged Cell division protein SepF C-terminal domain (2 mg/mL) from Mycobacterium tuberculosis

Cell division protein SepF
MWexperimental 23 kDa
MWexpected 25 kDa
VPorod 42 nm3
log I(s) 2.17×101 2.17×100 2.17×10-1 2.17×10-2
Cell division protein SepF small angle scattering data  s, nm-1
ln I(s)
Cell division protein SepF Guinier plot ln 2.18×101 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
Cell division protein SepF Kratky plot 1.104 0 √3 sRg
p(r)
Cell division protein SepF pair distance distribution function Rg: 2.5 nm 0 Dmax: 8.8 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of cell division protein SepF (C-terminal domain) in 25 mM Tris, 100 mM NaCl, 10% glycerol, 1 mM EDTA, pH 8.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 20°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Cell division protein SepF (SepF)
Mol. type   Protein
Organism   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Olig. state   Dimer
Mon. MW   12.4 kDa
 
UniProt   P9WGJ5 (123-218)
Sequence   FASTA