| MWexperimental | 12 | kDa |
| MWexpected | 15 | kDa |
| VPorod | 18 | nm3 |
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log I(s)
2.99×10-2
2.99×10-3
2.99×10-4
2.99×10-5
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s, nm-1
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Structural and functional repurposing of picornaviral H-box 2A proteins
Marion Pichon.SASDUY7 – Aichivirus A 2A protein at neutral pH
Genome polyprotein|
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Fits and models
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Synchrotron SAXS data from solutions of Aichivirus A 2A protein at neutral pH in 20 mM HEPES,150 mM NaCl, 2 mM TCEP, 1% v/v glycerol, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1549 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 40.00 μl sample at 10 mg/ml was injected at a 0.35 ml/min flow rate onto a GE Superdex 75 Increase 5/150 column at 20°C. 2880 successive 0.240 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Data were processed using the ATSAS suite. The high resolution structure of AiV-2A (chain A, B, C and A+B+C) theoretical scattering curve was fitted to the experimental data using rigid body and normal mode analysis (SREFLEX). CAUTION: The monomer component of the models (12.8 kDa) does not include all of the amino acids of the protein construct used for SAXS (14.6 kDa). |
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