Structural characterization of the interaction between BRCA1-BARD1 and RNA polymerase II

Tomas Klumpler.

SASDV28 – Breast cancer type 1 susceptibility protein, BRCA1 C-terminus domain (2 mg/ml)

Breast cancer type 1 susceptibility protein
MWexperimental 21 kDa
MWexpected 25 kDa
VPorod 34 nm3
log I(s) 5.73×10-1 5.73×10-2 5.73×10-3 5.73×10-4
Breast cancer type 1 susceptibility protein small angle scattering data  s, nm-1
ln I(s)
Breast cancer type 1 susceptibility protein Guinier plot ln 5.73×10-1 Rg: 2.3 nm 0 (2.3 nm)-2 s2
(sRg)2I(s)/I(0)
Breast cancer type 1 susceptibility protein Kratky plot 1.104 0 √3 sRg
p(r)
Breast cancer type 1 susceptibility protein pair distance distribution function Rg: 2.1 nm 0 Dmax: 5.9 nm

Data validation

There are no models related to this curve.

SAXS data from solutions of Breast cancer type 1 susceptibility protein, BRCA1 C-terminus domain in 25 mM Tris, 200 mM NaCl, 1 mM DTT, pH 7.9 were collected using a Rigaku BioSAXS-2000 instrument at CEITEC (Brno, Czech Republic) equipped with a HyPix-3000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 20°C. One 600 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Breast cancer type 1 susceptibility protein (BRCA1 BRCT)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Unknown
Mon. MW   25.0 kDa
 
UniProt   H0Y8B8 (500-717)
Sequence   FASTA