214-1

CHAO-CHENG CHO.

SASDV29 – Complex of human DNA methyltransferase 3 beta DNMT3B (215-853) and DNA methyltransferase 3-like DNMT3L (178-379)

DNA methyltransferase 3 beta (215-853)
DNA methyltransferase 3-like (178-379)
MWexperimental 192 kDa
MWexpected 192 kDa
VPorod 271263 nm3
log I(s) 1.68×10-1 1.68×10-2 1.68×10-3 1.68×10-4
DNA methyltransferase 3 beta (215-853) DNA methyltransferase 3-like (178-379) small angle scattering data  s, nm-1
ln I(s)
DNA methyltransferase 3 beta (215-853) DNA methyltransferase 3-like (178-379) Guinier plot ln 1.69×10-1 Rg: 5.2 nm 0 (5.2 nm)-2 s2
(sRg)2I(s)/I(0)
DNA methyltransferase 3 beta (215-853) DNA methyltransferase 3-like (178-379) Kratky plot 1.104 0 3 sRg
p(r)
DNA methyltransferase 3 beta (215-853) DNA methyltransferase 3-like (178-379) pair distance distribution function Rg: 5.4 nm 0 Dmax: 21.2 nm

Data validation


Fits and models


log I(s)
 s, nm-1
DNA methyltransferase 3 beta (215-853) DNA methyltransferase 3-like (178-379) GASBOR model

Synchrotron SAXS data from solutions of Complex of human DNA methyltransferase 3 beta DNMT3B (215-853) and DNA methyltransferase 3-like DNMT3L (178-379) in 20 mM Tris, 300 mM NaCl, 1 mM TCEP, 5% glycerol, pH 8 were collected on the 13A beam line at the Taiwan Photon Source, NSRRC storage ring (Hsinchu, Taiwan) using a Eiger X 1M and Eiger X 9M detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.0827 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 10°C. 162 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

DNA methyltransferase 3 beta (215-853) (DNMT3B (215-853))
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   72.6 kDa
 
UniProt   Q9UBC3 (215-853)
Sequence   FASTA
 
DNA methyltransferase 3-like (178-379) (DNMT3L (178-379))
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   23.6 kDa
 
UniProt   Q9UJW3 (178-379)
Sequence   FASTA