Polypyrimidine tract-binding protein 2

Estella Yee.

SASDV42 – Polypyrimidine tract-binding protein 2, full-length

Polypyrimidine tract-binding protein 2
MWexperimental 61 kDa
MWexpected 58 kDa
VPorod 98 nm3
log I(s) 3.25×100 3.25×10-1 3.25×10-2 3.25×10-3
Polypyrimidine tract-binding protein 2 small angle scattering data  s, nm-1
ln I(s)
Polypyrimidine tract-binding protein 2 Guinier plot ln 3.25×100 Rg: 4.2 nm 0 (4.2 nm)-2 s2
(sRg)2I(s)/I(0)
Polypyrimidine tract-binding protein 2 Kratky plot 1.104 0 √3 sRg
p(r)
Polypyrimidine tract-binding protein 2 pair distance distribution function Rg: 4.7 nm 0 Dmax: 17.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Polypyrimidine tract-binding protein 2 DAMMIN model
Synchrotron SAXS data from solutions of Polypyrimidine tract-binding protein 2, full-length in 25 mM Tris, 250 mM NaCl, 1 mM TCEP, pH 7.3 were collected on the 16-ID (LiX) beamline at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) using a Pilatus3 X 1M, Pilatus3 X 900K dual detectors at a sample-detector distance of 4.003 m (SAXS) and 0.282 m (WAXS) at a wavelength of λ = 0.08189 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 75.00 μl sample at 9 mg/ml was injected at a 0.35 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column. Five successive 1-second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Cell temperature = UNKNOWN. Storage temperature = UNKNOWN

Polypyrimidine tract-binding protein 2 (PTBP2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   57.9 kDa
 
UniProt   Q9UKA9 (1-531)
Sequence   FASTA