Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation

Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C, Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025) DOI

SASDV59 – Human Splicing factor, proline- and glutamine-rich protein 5% SFPQ and 95% dSFPQ at a D2O matchpoint of 95%

Splicing factor, proline- and glutamine-rich
MWexperimental 41 kDa
MWexpected 153 kDa
VPorod 181 nm3
log I(s) 2.85×10-2 2.85×10-3 2.85×10-4 2.85×10-5
Splicing factor, proline- and glutamine-rich small angle scattering data  s, nm-1
ln I(s)
Splicing factor, proline- and glutamine-rich Guinier plot ln 2.85×10-2 Rg: 6.1 nm 0 (6.1 nm)-2 s2
(sRg)2I(s)/I(0)
Splicing factor, proline- and glutamine-rich Kratky plot 1.104 0 3 sRg
p(r)
Splicing factor, proline- and glutamine-rich pair distance distribution function Rg: 6.6 nm 0 Dmax: 22 nm

Data validation


There are no models related to this curve.

SANS data from solutions of Human Splicing factor, proline- and glutamine-rich protein 5% SFPQ and 95% dSFPQ at a D2O matchpoint of 95% in 150 mM KCl, 0.075% glycerol, 20 mM HEPES, 0.5 mM DTT,, pH 7.4 were collected using a ORDELA/21000N detector at a sample-detector distance of 1.3 m and at a wavelength of λ = 0.6 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 25°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

SANS experiment indicating dimer partner exchange between deuterated and protiated monomers of full-length SFPQ. Contrast-matching has ensured signal is coming from protiated monomers of full-length SFPQ. Additional details are available in the manuscript: Structural dynamics of IDR interactions in human SFPQ and implications for liquid-liquid phase separation.

Splicing factor, proline- and glutamine-rich (SFPQ cysteine dimer)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   76.3 kDa
 
UniProt   P23246 (214-707)
Sequence   FASTA