| MWexperimental | 70 | kDa |
| MWexpected | 57 | kDa |
| VPorod | 131 | nm3 |
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log I(s)
2.89×10-2
2.89×10-3
2.89×10-4
2.89×10-5
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s, nm-1
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Diving into the structural architecture of the B2 SINE ribozyme
SASDV63 – B2 SINE ribozyme wildtype
B2 short interspaced nuclear element (SINE) RNA|
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Fits and models
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Synchrotron SAXS data from solutions of B2 WT RNA were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.094 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 1.6 mg/ml was injected onto a Shodex 403KW-4F HPLC column at 15°C using a flow rate of 0.16 ml/min. Eight successive 3 second frames were collected through the SEC elution peak of the sample (from a total of 600 SEC-SAXS data frames). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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