Apo Human APEX/APE-1

farheen jahan.

SASDV86 – Human DNA repair nuclease/redox regulator APEX1 (APE-1/APEX-1)

DNA repair nuclease/redox regulator APEX1
MWexperimental 36 kDa
MWexpected 36 kDa
VPorod 45 nm3
log I(s) 8.51×103 8.51×102 8.51×101 8.51×100
DNA repair nuclease/redox regulator APEX1 small angle scattering data  s, nm-1
ln I(s)
DNA repair nuclease/redox regulator APEX1 Guinier plot ln 8.51×103 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
DNA repair nuclease/redox regulator APEX1 Kratky plot 1.104 0 3 sRg
p(r)
DNA repair nuclease/redox regulator APEX1 pair distance distribution function Rg: 2.3 nm 0 Dmax: 8.3 nm

Data validation


Fits and models


log I(s)
 s, nm-1
DNA repair nuclease/redox regulator APEX1 DAMFILT model

SAXS data from solutions of Human DNA repair nuclease/redox regulator APEX1 (APE-1/APEX-1) in 50 mM HEPES pH 7.5, 200 mM NaCl were collected using an Anton Pair SAXSpace instrument at the CSIR-Central Drug Research Institute (Lucknow, India) equipped with a Mythen2 R 1K detector at a sample-detector distance of 0.317 and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 10°C. Two successive 1800 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Note: The model displayed in this entry is derived from the spatial alignment of an individual model cohort consisting of several different models where the aligned models have undergone subsequent volume and bead-occupancy correction (DAMFILT). Consequently, the model displayed in this entry is in no way related to the displayed fit to the data.

DNA repair nuclease/redox regulator APEX1
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   35.6 kDa
 
UniProt   P27695 (1-318)
Sequence   FASTA