Synchrotron SAXS
data from solutions of
The 60bp dsDNA oligo GAGE6_1
in
150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH 7.5
were collected
on the
SAXS/WAXS beam line
at the Australian Synchrotron storage ring
(Melbourne, Australia)
using a Pilatus3 S 2M detector
at a sample-detector distance of 2.4 m and
at a wavelength of λ = 0.10781 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 2.59 mg/ml was measured
at 25°C.
400 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The dsDNA duplex GAGE6_1 with the sequence:
5-GCCTTCTGCAAAGAAGTCTTGCGCATCGCGCGTGAAGTTTATTTCTAGCTTTTTGATGCT-3.
This measurement was performed at the SAXS/WAXS beamline of the Australian Synchrotron.
50µL of 70 µM sample in 150mM KCl, 20mM HEPES (pH 7.4), 5% glycerol, 5mM MgCl2, 1mM DTT was injected for the measurement.
This is part of the manuscript ' Probing the statistics of sequence-dependent DNA conformations in solution using SAXS '.
s, nm-1
