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SAXS data from solutions of Mycobacterium tuberculosis UvrA2.UvrB2 complex in 50 mM Tris pH 8, 200 mM NaCl, 2% glycerol were collected using an Anton Paar SAXSpace instrument at the CSIR-Central Drug Research Institute (Lucknow India) equipped with a Mythen2 R 1K detector at a sample-detector distance of 0.320 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.90 mg/ml was measured at 10°C. Two successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Note: Data are of very-low quality. The experimental molecular weight cannot be obtained/confirmed from the SAXS data data. Due to the low concentration, the data contains noise in the sample—the complex of Mtb. UvrA dimer and UvrB dimer were purified by co-expressing His-tagged Mtb. UvrA and untagged Mtb.UvrB protein in BL-21 cells. The untagged UvrB forms a complex with UvrA (pre-initiation complex) and is purified. Further purification was performed by size exclusion chromatography using a superose 6 10/300 GL column, which shows the protein's equimolar and homogenous solution—the calculated molecular weight of the Mtb. UvrA2B2 complex using SEC is 343kDa.
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s, nm-1
