High mobility group protein B2 (HMGB2)

Chun I Tu.

SASDV89 – Mouse High mobility group protein B2 HMGB2-dsDNA complex

High mobility group protein B2
Double stranded DNA 20mer
MWexperimental 35 kDa
MWexpected 38 kDa
VPorod 50 nm3
log I(s) 7.32×10-2 7.32×10-3 7.32×10-4 7.32×10-5
High mobility group protein B2 Double stranded DNA 20mer small angle scattering data  s, nm-1
ln I(s)
High mobility group protein B2 Double stranded DNA 20mer Guinier plot ln 7.33×10-2 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
High mobility group protein B2 Double stranded DNA 20mer Kratky plot 1.104 0 √3 sRg
p(r)
High mobility group protein B2 Double stranded DNA 20mer pair distance distribution function Rg: 3.3 nm 0 Dmax: 12.2 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Mouse High mobility group protein B2 HMGB2-dsDNA complex in Tris 50mM, NaCl 150mM, pH 7 were collected on the 13A beam line at the Taiwan Photon Source, NSRRC storage ring (Hsinchu, Taiwan) using a Eiger X 1M and Eiger X 9M detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.08265 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 10°C. 148 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

High mobility group protein B2
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   25.8 kDa
 
UniProt   P30681 (1-210)
Sequence   FASTA
 
Double stranded DNA 20mer
Mol. type   DNA
Olig. state   Monomer
Mon. MW   12.4 kDa
Sequence   FASTA