Leishmania donovani Trypanothione Reductase (LdTR) protein

Karthik Ramalingam.

SASDV96 – Trypanothione Reductase, Leishmania donovani protein

Trypanothione reductase
MWexperimental 110 kDa
MWexpected 108 kDa
VPorod 180 nm3
log I(s) 3.22×104 3.22×103 3.22×102 3.22×101
Trypanothione reductase small angle scattering data  s, nm-1
ln I(s)
Trypanothione reductase Guinier plot ln 3.22×104 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Trypanothione reductase Kratky plot 1.104 0 √3 sRg
p(r)
Trypanothione reductase pair distance distribution function Rg: 3.2 nm 0 Dmax: 9.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Trypanothione reductase PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Trypanothione reductase DAMFILT model
SAXS data from solutions of Leishmania donovani trypanothione reductase in 50 mM Tris, pH 8 were collected using an Anton Pair SAXSpace instrument at the CSIR-Central Drug Research Institute (Lucknow, India) equipped with a Mythen2 R 1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 10°C. Two successive 1800 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Note: The dummy atom model (DAM) displayed in this entry is derived from the spatial alignment of an individual model cohort consisting of several different models where the aligned models have undergone subsequent volume and bead-occupancy correction (DAMFILT). Consequently, the DAM model displayed in this entry is in no way related to the displayed fit to the data.

Trypanothione reductase
Mol. type   Protein
Organism   Leishmania donovani donovani
Olig. state   Dimer
Mon. MW   53.9 kDa
 
UniProt   Q95PT6 (1-491)
Sequence   FASTA
 
PDB ID   1FEC