High mobility group protein B2 (HMGB2)

Chun I Tu.

SASDV99 – Mouse High mobility group protein B2 (HMGB2) apo form

High mobility group protein B2
MWexperimental 28 kDa
MWexpected 26 kDa
VPorod 36 nm3
log I(s) 4.39×10-2 4.39×10-3 4.39×10-4 4.39×10-5
High mobility group protein B2 small angle scattering data  s, nm-1
ln I(s)
High mobility group protein B2 Guinier plot ln 4.40×10-2 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
High mobility group protein B2 Kratky plot 1.104 0 √3 sRg
p(r)
High mobility group protein B2 pair distance distribution function Rg: 2.9 nm 0 Dmax: 10.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Mouse High mobility group protein B2 (HMGB2) apo form Rg histogram Rg, nm
Synchrotron SAXS data from solutions of Mouse High mobility group protein B2 (HMGB2) apo form in Tris 50mM, NaCl 150mM, pH 7 were collected on the 13A beam line at the Taiwan Photon Source, NSRRC storage ring (Hsinchu, Taiwan) using a Eiger X 1M and Eiger X 9M detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.08265 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 10°C. 124 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

High mobility group protein B2
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   25.8 kDa
 
UniProt   P30681 (1-210)
Sequence   FASTA