Synchrotron SAXS
data from solutions of
The 60bp dsDNA oligo GAGE6_3
in
150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH 7.5
were collected
on the
SAXS/WAXS beam line
at the Australian Synchrotron storage ring
(Melbourne, Australia)
using a Pilatus3 S 2M detector
at a sample-detector distance of 2.4 m and
at a wavelength of λ = 0.10781 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 3.01 mg/ml was measured
at 25°C.
400 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The 60bp dsDNA duplex GAGE6_3:
5-GCCTTCTGCAAAGAAGTCTTGCGCATCGCGCGTGAAGGCGCGCGCTAGCGCGCGGATGCT-3
This data was collected at the Australian Synchrotron by injecting 50 µL of sample at 81 µM in 150mM KCl, 20mM HEPES (pH 7.4), 5% glycerol, 5mM MgCl2, 1mM DTT.
This data is part of the manuscript: Probing the statistics of sequence-dependent DNA
conformations in solution using SAXS.
s, nm-1
