High mobility group protein B2 (HMGB2)

Chun I Tu.

SASDVB9 – C-terminally truncated form of mouse High mobility group protein B2 (HMGB2ΔC)-dsDNA complex

High mobility group protein B2
MWexperimental 31 kDa
MWexpected 20 kDa
VPorod 42 nm3
log I(s) 1.41×10-1 1.41×10-2 1.41×10-3 1.41×10-4
High mobility group protein B2 small angle scattering data  s, nm-1
ln I(s)
High mobility group protein B2 Guinier plot ln 1.41×10-1 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
High mobility group protein B2 Kratky plot 1.104 0 √3 sRg
p(r)
High mobility group protein B2 pair distance distribution function Rg: 2.9 nm 0 Dmax: 10.4 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of C-terminally truncated form of mouse High mobility group protein B2 (HMGB2ΔC)-dsDNA complex in Tris 50mM, NaCl 150mM, pH 7 were collected on the 13A beam line at the Taiwan Photon Source, NSRRC storage ring (Hsinchu, Taiwan) using a Eiger X 1M and Eiger X 9M detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.08265 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 10°C. 147 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

High mobility group protein B2 (HMGB2 tail truncated)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   20.2 kDa
 
UniProt   P30681 (1-166)
Sequence   FASTA