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Synchrotron SAXS data from solutions of DNA repair nuclease/redox regulator APEX1 (APE-1/APEX-1) bound to DNA ligase-1 in 50 mM Tris pH 7.5, 200 mM NaCl, 2% glycerol were collected on the BL-18 beam line at the INDUS-2 (Indore, India) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 25°C. Two successive 1800 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
CAUTION: Dmax underestimated. CAUTION: Likely inconsistency in background subtraction. Note: X-ray wavelength= UNKNOWN. Note: Sample to detector distance = UNKNOWN. Note: The model displayed in this entry is derived from the spatial alignment of an individual model cohort consisting of several different models where the aligned models have undergone subsequent volume and bead-occupancy correction (DAMFILT). Consequently, the model displayed in this entry is in no way related to the displayed fit to the data.
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s, nm-1
