Synchrotron SAXS
data from solutions of
Probable chromosome-partitioning protein ParB bound to parS1 DNA (vcParB1-parS1-peak)
in
20 mM Tris pH 7.5,100 mM NaCl, 2% glycerol, 1 mM EDTA, 2 mM DTT, 5 mM MgCl2
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus 1M detector
at a sample-detector distance of 2.8 m and
at a wavelength of λ = 0.0999 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample
at 4.3 mg/ml was injected at a 0.07 ml/min flow rate
onto a GE Superdex 200 Increase 3.2/300 column
at 20°C.
960 successive
2 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
SEC-SAXS run on vcParB1-parS1 nucleoprotein complex
s, nm-1
