Hierarchy in regulator interactions with distant transcriptional activation domains empowers rheostatic regulation

Due A, Davey N, Thomasen F, Morffy N, Prestel A, Brakti I, O'Shea C, Strader L, Lindorff‐Larsen K, Skriver K, Kragelund B, Protein Science 34(6) (2025) DOI

SASDVP9 – NAC domain-containing protein 46 (ANAC046) Intrinsically disordered region (172-338) in a 1:1 molar ratio binding with Inactive poly [ADP-ribose] polymerase RCD1-RST (499-572) from A.thaliana at pH 7.0

NAC domain-containing protein 46
Inactive poly [ADP-ribose] polymerase RCD1
MWexperimental 22 kDa
MWexpected 26 kDa
VPorod 38 nm3
log I(s) 2.46×103 2.46×102 2.46×101 2.46×100
NAC domain-containing protein 46 Inactive poly [ADP-ribose] polymerase RCD1 small angle scattering data  s, nm-1
ln I(s)
NAC domain-containing protein 46 Inactive poly [ADP-ribose] polymerase RCD1 Guinier plot ln 2.46×103 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
NAC domain-containing protein 46 Inactive poly [ADP-ribose] polymerase RCD1 Kratky plot 1.104 0 √3 sRg
p(r)
NAC domain-containing protein 46 Inactive poly [ADP-ribose] polymerase RCD1 pair distance distribution function Rg: 4.2 nm 0 Dmax: 20.6 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of NAC domain-containing protein 46 (ANAC046) Intrinsically disordered region (172-338) in a 1:1 molar ratio binding with Inactive poly [ADP-ribose] polymerase RCD1-RST (499-572) from A.thaliana at pH 7.0 in 20mM Na2HPO4/NaH2PO4, 100mM NaCl, 5mM DTT, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123982 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 25°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN. Concentration = UNKNOWN

NAC domain-containing protein 46 (ANAC046)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Monomer
Mon. MW   18.0 kDa
 
UniProt   Q9SQQ6 (172-338)
Sequence   FASTA
 
Inactive poly [ADP-ribose] polymerase RCD1 (RCD1-RST)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Monomer
Mon. MW   8.5 kDa
 
UniProt   Q8RY59 (499-572)
Sequence   FASTA