Hierarchy in regulator interactions with distant transcriptional activation domains empowers rheostatic regulation

Due A Davey N, Thomasen F, Morffy N, Prestel A, Brakti I, O'Shea C, Strader L, Lindorff‐Larsen K, Skriver K, Kragelund B, Protein Science 34(6) (2025) DOI

SASDVP9 – NAC domain-containing protein 46 (ANAC046) Intrinsically disordered region (172-338) in a 1:1 molar ratio binding with Inactive poly [ADP-ribose] polymerase RCD1-RST (499-572) from A.thaliana at pH 7.0

NAC domain-containing protein 46
Inactive poly [ADP-ribose] polymerase RCD1
MWexperimental 22 kDa
MWexpected 26 kDa
VPorod 38 nm3
log I(s) 2.46×103 2.46×102 2.46×101 2.46×100
NAC domain-containing protein 46 Inactive poly [ADP-ribose] polymerase RCD1 small angle scattering data  s, nm-1
ln I(s)
NAC domain-containing protein 46 Inactive poly [ADP-ribose] polymerase RCD1 Guinier plot ln 2.46×103 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
NAC domain-containing protein 46 Inactive poly [ADP-ribose] polymerase RCD1 Kratky plot 1.104 0 √3 sRg
p(r)
NAC domain-containing protein 46 Inactive poly [ADP-ribose] polymerase RCD1 pair distance distribution function Rg: 4.2 nm 0 Dmax: 20.6 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of NAC domain-containing protein 46 (ANAC046) Intrinsically disordered region (172-338) in a 1:1 molar ratio binding with Inactive poly [ADP-ribose] polymerase RCD1-RST (499-572) from A.thaliana at pH 7.0 in 20mM Na2HPO4/NaH2PO4, 100mM NaCl, 5mM DTT, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123982 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 25°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN. Concentration = UNKNOWN

NAC domain-containing protein 46 (ANAC046)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Monomer
Mon. MW   18.0 kDa
 
UniProt   Q9SQQ6 (172-338)
Sequence   FASTA
 
Inactive poly [ADP-ribose] polymerase RCD1 (RCD1-RST)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Monomer
Mon. MW   8.5 kDa
 
UniProt   Q8RY59 (499-572)
Sequence   FASTA