| MWexperimental | 44 | kDa |
| MWexpected | 42 | kDa |
| VPorod | 65 | nm3 |
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log I(s)
2.23×100
2.23×10-1
2.23×10-2
2.23×10-3
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s, nm-1
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Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky.SASDVQ8 – Glucosidase/Rutinosidase from Penicillium rubens - deglycosylated
glucan 1,3-beta-glucosidase|
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Fits and models
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SAXS data from solutions of Glucosidase/Rutinosidase (deglycosylated) in 50 mM sodium citrate, 150 mM NaCl, pH 4.5 were collected using an Anton Paar SAXSpoint 2.0 instrument at the Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure (Vestec, Czech Republic) equipped with an Eiger R 1M detector at a sample-detector distance of 0.8 m and at a wavelength of λ = 0.134 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 20°C. 60 successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Glucosidase/Rutinosidase from Penicillium rubens was deglycosylated using endo H glycosidase. The data were measured using ASX autosampler and SiN cell. Data may be oversubtracted between 2.70 < s < 2.96 nm-1. |
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